Nucleocapsid mutations gave delta its ability to replicate 10X faster

Login or register to post comments Last Post 0 reads   10 posts
  • Fri, Nov 05, 2021 - 11:41am

    #1
    Disco Bear

    Disco Bear

    Status Gold Member (Offline)

    Joined: Dec 16 2020

    Posts: 297

    count placeholder2

    Nucleocapsid mutations gave delta its ability to replicate 10X faster

While all eyes have been focused on mutations to the spike protein, mutations to the nucleocapsid of the delta variant may explain why its viral load explodes so quickly.

From the Abstract on https://www.science.org/doi/10.1126/science.abl6184 :

In SC2-VLPs, four nucleocapsid (N) mutations found universally in more-transmissible variants independently increased mRNA delivery and expression by ~10-fold, and in a reverse genetics model, S202R and R203M each produced >50-fold more virus.

For those of you who aren’t experts in virology, a less technical explanation is give on https://www.dailymail.co.uk/news/article-10168639/Is-Delta-infectious-Scientists-little-known-mutation-Covid-variant.html .

This discovery could turn out to be minor or extremely important.  Playing around with altering the vaccines to target spike protein mutations more accurately may result in very little improvement in their ability to halt the pandemic if the key mutation is on the nucleocapsid.   It is my limited understanding that the nucleocapsid is not on the outside of the virus particle, thus cannot be recognized by antibodies because they do not have access to it, thus these mutations which are making the virus replicate more quickly cannot be defeated by vaccinations at all. [Hint: early treatments will be the only successful strategy against a rapidly replicating virus.  Waiting until hospitalization to begin treatment is tantamount to a death sentence for many and will guarantee that the virus circulates in society with impunity.]

I’m hoping more knowledgeable members of Peak Prosperity than myself, a retired accountant, will discuss this further and confirm or deny my hypothesis.  I’m way out of my expertise when discussing this.  Let me know if I’m full of s**t if that is the case.  It won’t bother me — my ego was destroyed decades ago.  I just want the truth.  If my understanding of the structure of the virus is in error, then it is likely that my error is shared by many others.  Correcting that error will improve understanding for everybody.

Here’s a diagram of the virus showing how no part of the nucleocapsid is located on the surface:

image source: https://www.invivogen.com/sites/default/files/invivogen/categories/images/figures/structure-proteins-covid-19-invivogen.png found on https://www.invivogen.com/sars2-nucleocapsid-expression-vector

  • Fri, Nov 05, 2021 - 02:57pm

    #2
    XZBD2

    XZBD2

    Status Bronze Member (Offline)

    Joined: Apr 15 2020

    Posts: 87

    count placeholder2

    Nucleocapsid mutations gave delta its ability to replicate 10X faster

That is an important finding. Thanks for sharing.

There are certainly many more qualified to answer this then myself, but I believe in a natural response to infection antibodies to the nucleocapsid protein are actually quite prevalent and as this area is one of the more conserved there is even cross reactivity from previous Coronavirus infections to the nuclear capsid proteins. In an over simplification it is because since viruses replicate inside cells and antibodies are outside cells the way our body gets around this problem is that cells display whatever they are producing on their surface.  If a T cell comes along and identifies this displayed protein on the surface as foreign it will activate the compliment system and that cell is destroyed and thus its contents released including all the virus pieces that were under production and not yet packaged in an envelope. Antibodies IgG if the content is released into the blood or IgA if into the mucosal layer can then interact and bind to the released parts.  This all leads to a cascade that triggers typical symptoms like runny nose, inflammation and fever warning adjacent cells while cleaning up the debris.  Sars Cov-2 produces proteins that are designed to block this process, which is why people remain asymptomatic for part or all of the infection.  This is one of the reasons Ivermectin is so important as it blocks the blockers thus the alert can be sent.

  • Fri, Nov 05, 2021 - 03:42pm   (Reply to #2)

    #3
    XZBD2

    XZBD2

    Status Bronze Member (Offline)

    Joined: Apr 15 2020

    Posts: 87

    count placeholder0

    Nucleocapsid mutations gave delta its ability to replicate 10X faster

Here is a study demonstrating preexisting antibodies to the nucleocapsid protein:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8062931/

 

  • Sat, Nov 06, 2021 - 11:34am   (Reply to #2)

    #4
    Disco Bear

    Disco Bear

    Status Gold Member (Offline)

    Joined: Dec 16 2020

    Posts: 297

    count placeholder0

    Dr. Marik says spike protein cannot be expressed on the surface of a cell

In post #2, XZBD2 typed

cells display whatever they are producing on their surface.

I have encountered that concept being used by others.

In contrast, Dr. Paul Marik says there is no mechanism for the spike protein to be expressed on the surface of an individual cell.  He says the idea is “total and utter garbage.”  He gives a very interesting rant that starts around 35:15 in the FLCCC weekly update 28 July 2021.

Comments on explaining this difference of opinion would be appreciated!

  • Sat, Nov 06, 2021 - 04:36pm

    #5
    tbp

    tbp

    Status Platinum Member (Offline)

    Joined: Apr 12 2020

    Posts: 1123

    count placeholder0

    Nucleocapsid mutations gave delta its ability to replicate 10X faster

^ The Spike proteins are not really spikey/sharp, so I think they won’t protrude thru a cell membrane unless there’s some mechanism such as receptor binding for it to happen. Normally cell “janitors” like L-carnitine will carry debris out, or for larger objects phagocytes will engulf/eat and then dissolve into smaller pieces that can then be removed from the cell, or carry them first out of the cell.


@Disco
Bear, interesting hypothesis! Would this have happened without the Enhanced-Wuhan-Spike protein injections that attenuate/destroy the natural Nucleocapsid-based antibodies? My guess is NO WAY, i.e. Delta Spike and Nucleocapsid mutations are a direct consequence of the “vaccine” bioweapon injections. Particularly as the Nucleocapsid protein is now more free to proliferate (and thus hCoVs may no longer have highly conserved Nucleocapsids?). Could it be directly intended, i.e. did they know this would happen?

  • Sat, Nov 06, 2021 - 08:32pm   (Reply to #5)

    #6
    Disco Bear

    Disco Bear

    Status Gold Member (Offline)

    Joined: Dec 16 2020

    Posts: 297

    count placeholder0

    Dr. Richard Fleming says the opposite of Dr. Paul Marik.

In post #5 tbp typed

so I think they won’t protrude thru a cell membrane unless there’s some mechanism such as receptor binding for it to happen.

Listen to Dr. Richard Fleming say the following @ 01:48 in the video entitled “How The Vaxx is Breaking Down the Body” :

and it too is then presented to the surface of our cells.

That sounds like a direct contradiction to what Dr. Paul Marik says in post #2.  I have a suspicion that Dr. Fleming is leaving out some step in this process of presenting the spike protein to the surface of our cells, because earlier he mentions enzymes acting on the spike protein before they are presented to the surface of the cell.  Can somebody please explain what is going on here?

I forget the name of the doctor, I believe he is from Canada in a town that got hit hard by the forest fires, made a video claiming the spike proteins would be expressed on the cells of the lining of blood vessels, giving it a rough appearance like sandpaper.  If I remember correctly, that rough appearance would encourage the formation of blood clots.  It may be that vivid, but somewhat inaccurate picture, is what upset Dr. Marik.

  • Sat, Nov 06, 2021 - 09:17pm   (Reply to #5)

    #7
    westcoastjan

    westcoastjan

    Status Platinum Member (Offline)

    Joined: Jun 04 2012

    Posts: 1463

    count placeholder1

    Nucleocapsid mutations gave delta its ability to replicate 10X faster

The Canadian doctor you are referring to is Dr. Hoffe

I don’t have a horse in this race, but Dr. Fleming is a nuclear cardiologist, PhD physicist (as well as a lawyer), so I think he more qualified to speak to this than Dr. Marik. Many of Dr. Fleming’s videos have been featured here at PP. I highly recommend his Masterclass on covid, which while on the long side, is most excellent at explaining things in a way most laypeople can understand.

  • Sun, Nov 07, 2021 - 11:18pm

    #8
    Island girl

    Island girl

    Status Silver Member (Offline)

    Joined: Nov 27 2017

    Posts: 356

    count placeholder1

    Nucleocapsid mutations gave delta its ability to replicate 10X faster

This finding is quite concerning. Can someone please confirm whether the nucleocapsid protein is the so-called N protein that has been proposed to generate immune memory in natural infection but not in those infected post-vaccination? What role does it play in the organism?

 

  • Mon, Nov 08, 2021 - 05:49am   (Reply to #8)

    #9
    Disco Bear

    Disco Bear

    Status Gold Member (Offline)

    Joined: Dec 16 2020

    Posts: 297

    count placeholder0

    The nucleocapsid protein is the so-called N protein

Island girl, the nucleocapsid protein and the N protein are the same thing.  I vaguely remember seeing at least one article suggesting that vaccination might suppress the immune system’s ability to generate antibodies to the N protein, but as this is not my area of expertise, I can’t agree or disagree with that concept.

Excerpt from https://www.nature.com/articles/s41598-021-83108-0 :

SARS-CoV-2 has four key structural proteins. The Nucleocapsid Protein (N Protein) is found in the viral core3. The Spike Protein (S Protein), Matrix Protein (M Protein) and Envelope Protein (E Protein)3 are all found on the virus’s outer surface.

. . .

Although the functionality of the N protein is not well understood, it has been well studied in SARS-CoV18. The N protein is closely associated with viral RNA structure and function and plays a vital role in viral transcription and assembly while functioning as an RNA chaperone. The N segment of the RNA has 3 distinct regions; a motif responsible for length of amino acids of N protein, a sumoylation motif (lysin residue), and a serin residue responsible for phosphorylation by a cyclin-dependent kinase complex.

  • Mon, Nov 08, 2021 - 06:22am   (Reply to #5)

    #10
    Disco Bear

    Disco Bear

    Status Gold Member (Offline)

    Joined: Dec 16 2020

    Posts: 297

    count placeholder0

    Dr. Hoffe’s town of Lytton 90% destroyed by fire.

Thank you, Westcoastjan, for supplying Dr. Hoffe’s name.  Lytton was almost completely destroyed by fire as this video shows.

Dr. Hoffe’s clinic is now permanently closed, no doubt as a result of the fire.  It was located in the heart of Lytton.

Viewing 10 posts - 1 through 10 (of 10 total)

Login or Register to post comments